ʻO ka hoʻāʻo, i hoʻomohala ʻia e nā ʻenekini biomedical a me nā virologists mai ke Kulanui o Glasgow, e hāʻawi i nā hopena lateral-flow e like me ka hoʻāʻo home COVID-19 ma kahi o 30 mau minuke.
Ma kahi pepa hou i paʻi ʻia i kēia lā ma ka puke pai Nature Communications, wehewehe ka hui noiʻi pehea lākou i hoʻomohala ai i ka ʻōnaehana. Kūkulu ʻia ia ma luna o nā holomua mua i ka diagnostics wikiwiki a me ka virology ma ke Kulanui, e hāʻawi ana i nā hopena me 98% pololei.
ʻO ka maʻi hepatitis C, he maʻi koko e hoʻopōʻino i ke ake, ua manaʻo ʻia e hoʻopilikia i ka 70 miliona mau kānaka a puni ka honua. He lohi ka hopena o ka maʻi maʻi ma ke akepaʻa, a ʻaʻole ʻike ka poʻe maʻi i ka maʻi a hiki i ka wā e maʻi nui ai lākou me nā pilikia e like me ka cirrhosis a i ʻole ka maʻi kanesa.
Inā ʻike ʻia ka maʻi ma mua o ka piʻi nui ʻana, hiki ke mālama maikaʻi ʻia me ka lāʻau haʻahaʻa haʻahaʻa a hiki ke loaʻa. Eia nō naʻe, ʻo ka nui o ka 80 pakeneka o ka poʻe me ka maʻi maʻi ʻaʻole ʻike i ko lākou maʻi a hiki i ka wā e hiki mai ai nā pilikia maʻi.
ʻO ka hopena, ma kahi o 400,000 poʻe a puni ka honua e make i nā maʻi hepa C i kēlā me kēia makahiki, a ʻo ka hapa nui o lākou i hiki ke hoʻopakele ʻia e ka maʻi ma mua a me ka mālama ʻana.
I kēia manawa, ʻike ʻia ka maʻi hepatitis C i loko o nā kūlana hoʻokolohua me ka hoʻohana ʻana i kahi kaʻina hana ʻelua e hoʻāʻo ai i ke koko no ka loaʻa ʻana o nā antibodies a me ka ʻike ʻana i ka maʻi 'RNA a i ʻole nā antigens kumu.
Hiki i ke kaʻina hana ke lawe i ka manawa nui no ka hoʻopuka ʻana i nā hopena, e hoʻonui ana i ka hiki ke hoʻi mai kekahi poʻe maʻi e lawe i ka hoʻokolohua e aʻo i ka hopena. Ua kaupalena ʻia ke komo ʻana i nā hoʻokolohua ma nā ʻāina haʻahaʻa a me waena, kahi e noho ai ka hapa nui o ka poʻe me ka maʻi hepa C.
ʻOiai ua hoʻomohala ʻia nā hoʻokolohua ʻoi aku ka hiki ke hoʻopuka i nā hopena wikiwiki i nā makahiki i hala iho nei, hiki ke kaupalena ʻia ko lākou pololei, ʻoi aku ma waena o nā genotypes kanaka.
ʻO ka ʻōnaehana hou a ke Kulanui o Glasgow i alakaʻi ʻia, ʻoi aku ka maikaʻi-pono no ka hoʻohana ʻana a puni ka honua. Hoʻololi ʻia ia mai kahi ʻōnaehana like a lākou i kūkulu ai e hoʻopuka i ka maʻi maʻi maʻi malaria, i hoʻāʻo ʻia me nā hopena hoʻoikaika ma Uganda.
Hoʻohana ka hāmeʻa i nā ʻāpana o ka pepa wax me like me ka origami e hoʻomākaukau ai i nā laʻana no kahi kaʻina hana i kapa ʻia ʻo loop-mediated isothermal amplification, a i ʻole LAMP. ʻO ke kaʻina hana o ka peʻe pepa e hiki ai ke hana a hāʻawi ʻia i ʻekolu keʻena liʻiliʻi i loko o kahi pahu pahu, kahi e hoʻomehana ai ka mīkini LAMP a hoʻohana e hoʻāʻo i nā laʻana no ka loaʻa ʻana o ka hepatitis C RNA. He mea maʻalahi ka ʻenehana i hiki ke hāʻawi ʻia i ka wā e hiki mai ana, mai kahi laʻana koko i lawe ʻia mai ka mea maʻi ma o ka manamana lima.
He 30 mau minuke ke kaʻina hana. Hāʻawi ʻia nā hopena ma o ka maʻalahi o ka heluhelu ʻana i ka lateral flow strip e like me ka hoʻāʻo hāpai ʻana a i ʻole ka hoʻāʻo home COVID-19, e hōʻike ana i ʻelua mau pūʻali no ka hopena maikaʻi a hoʻokahi band no ka maikaʻi ʻole.
No ka hoʻāʻo ʻana i kā lākou prototype, ua hoʻohana ka hui i ka ʻōnaehana e kālailai i 100 anonymised blood plasma samples mai nā maʻi me kahi maʻi HCV mau a me kekahi mau laʻana 100 mai nā maʻi HCV-negative, i hana ma ke ʻano he pūʻulu hoʻomalu. Ua hoʻāʻo pū ʻia nā laʻana me ka hoʻohana ʻana i ka ʻoihana maʻamau Abbott RealTime hepatitis C assay e hōʻoia i nā hopena LAMP. Hāʻawi nā hōʻike LAMP i nā hopena he 98% pololei.
Ke manaʻo nei ka hui e hoʻohana i ka ʻōnaehana i nā hoʻokolohua kahua ma sub-Saharan Africa i ka makahiki aʻe.
Ua paʻi ʻia ka pepa a ka hui, ʻo 'Loop mediated isothermal amplification ma ke ʻano he mea hana ikaika no ka ʻike mua ʻana o ka maʻi hepatitis C', ua paʻi ʻia ma Nature Communications. Ua kākoʻo ʻia ka noiʻi ʻana e ke kālā mai ka ʻEnekinia a me ka ʻEpekema ʻEpekema Research Council (EPSRC), Medical Research Council a me ka Wellcome Trust.
He aha e lawe ʻia mai kēia ʻatikala:
- The process of paper folding enables the sample to be processed and delivered to three small chambers in a cartridge, which the LAMP machine heats and uses to test the samples for the presence of hepatitis C RNA.
- The technique is sufficiently simple that it has the potential, in future, to be delivered in the field, from a blood sample taken from a patient via fingerprick.
- The results are delivered through an easy-to-read lateral flow strip like a pregnancy test or a home COVID-19 test, which shows two bands for a positive result and one band for a negative.
