ʻO ka hoʻolaha wikiwiki ʻana o COVID-19, kahi maʻi i hoʻokumu ʻia e ka maʻi SARS-CoV-2, ua hoʻokumu i kahi pilikia olakino lehulehu a puni ka honua. ʻO ka ʻike mua ʻana a me ka hoʻokaʻawale ʻana i ka COVID-19 he kī nui no ka pale ʻana i ka hoʻoili ʻana o ka maʻi a me ka pale ʻana i nā heluna nāwaliwali. ʻO ka maʻamau o kēia manawa no ka maʻi maʻi COVID-19 he reverse transcriptase-polymerase chain reaction (RT-PCR), kahi ʻenehana e ʻike ʻia ai nā ʻano viral ma hope o ka loaʻa ʻana o nā pōʻai he nui o ka amplification. Eia nō naʻe, hoʻopau manawa kēia ʻenehana, e hana ana i kahi backlog hoʻāʻo ma waena o nā kikowaena diagnostic a alakaʻi i nā diagnoses lohi.

I loko o kahi noiʻi hou i paʻi ʻia ma Biosensors and Bioelectronics, ua hoʻopuka nā mea noiʻi mai Korea a me Kina i kahi papahana nanotechnology-based platform e hiki ke hōʻemi i ka manawa e pono ai no ka maʻi COVID-19. Ko lakou 'ilikai-hoonui Raman hoopuehu (SERS)-PCR ike anuu-i hoomakaukauia me ke gula nanoparticles (AuNPs) i loko o ka cavities o Au 'nanodimple' substrates (AuNDSs) - hiki ke ike viral genes ma hope o 8 wale pōʻaiapuni o ka amplification. ʻO ia kahi kokoke i kahi hapakolu o ka helu i koi ʻia me ka RT-PCR maʻamau.

"Hoʻokumu ʻia ka RT-PCR maʻamau i ka ʻike ʻana i nā hōʻailona fluorescence, no laila pono 3-4 mau hola e ʻike ai iā SARS-CoV-2. ʻAʻole lawa kēia wikiwiki e noʻonoʻo i ka wikiwiki o ka laha ʻana o COVID-19. Ua makemake mākou e ʻimi i kahi ala e ʻoki ai i kēia manawa ma ka hapa liʻiliʻi, "wahi a Prof. Jaebum Choo, e wehewehe ana i ke kumu o ke aʻo ʻana. ʻO ka mea pōmaikaʻi, ʻaʻole i mamao loa ka pane. Ma kahi noiʻi mua i paʻi ʻia ma 2021, ua hoʻomohala ka hui o Prof. Choo i kahi kahua ʻike noʻonoʻo kahi e hana ʻia ai nā hōʻailona SERS kiʻekiʻe e nā AuNP i hoʻonohonoho like ʻia i loko o nā lua o AuNDS ma o kahi ʻenehana i kapa ʻia ʻo DNA hybridization. Ma muli o kēia ʻike mua, ua hoʻomohala ʻo Prof. Choo a me kāna hui i ka moʻolelo SERS-PCR platform no ka maʻi COVID-19.

Ke hoʻohana nei ka SERS-PCR assay hou i nā hōʻailona SERS no ka ʻike ʻana i ka "DNA bridge" - nā ʻimi DNA liʻiliʻi e haki mālie ana i mua o nā genes viral target. No laila, i nā laʻana mai nā mea maʻi maikaʻi no COVID-19, ke emi mau nei ka neʻe ʻana o ke alahaka DNA (a no laila ka hōʻailona SERS) me nā holomua PCR holomua. I ka hoʻohālikelike ʻana, i ka wā i hala ʻole ai ʻo SARS-CoV-2, ʻaʻole i loli ka hōʻailona SERS.

Ua hoʻāʻo ka hui i ka maikaʻi o kā lākou ʻōnaehana me ka hoʻohana ʻana i ʻelua mau māka makaʻāinana o SARS-CoV-2, ʻo ia hoʻi, ka envelope protein (E) a me RNA-dependent RNA polymerase (RdRp) genes o SARS-CoV-2. ʻOiai ua koi ʻia nā pōʻai he 25 no ka ʻike ʻana i ka RT-PCR, ʻo ka paepae SERS-PCR i hoʻokumu ʻia ma AuNDS e koi i nā pōʻai 8 wale nō, e hōʻemi nui ana i ka lōʻihi o ka hoʻāʻo. "ʻOiai he mea mua kā mākou hopena, hāʻawi lākou i kahi hōʻoia koʻikoʻi o ka manaʻo no ka pono o SERS-PCR ma ke ʻano he ʻano diagnostic. ʻO kā mākou ʻenehana SERS-PCR i hoʻokumu ʻia ma AuNDS he kahua hoʻopōmaikaʻi molecular diagnostic hou e hiki ke hoʻopōkole i ka manawa i koi ʻia no ka ʻike ʻana i ke ʻano i hoʻohālikelike ʻia me nā ʻenehana RT-PCR maʻamau. Hiki ke hoʻonui hou ʻia kēia ʻano hoʻohālike ma o ka hoʻokomo ʻana i kahi mea hoʻohālike maʻamau e hoʻomohala i kahi ʻōnaehana diagnostic molecular e hiki mai ana," wahi a Prof. Choo.

ʻOiaʻiʻo, hiki i ka SERS-PCR ke lilo i mea hana koʻikoʻi i kā mākou arsenal e kūʻē i ka maʻi maʻi COVID-19. Hiki iā ia ke hana i kahi hoʻololi paradigm i ke kahua o ka diagnostics molecular, hoʻololi i ke ʻano o kā mākou ʻike ʻana i nā maʻi maʻi a hoʻoponopono i nā maʻi maʻi e hiki mai ana.

He aha e lawe ʻia mai kēia ʻatikala:

In a recent study published in Biosensors and Bioelectronics, researchers from Korea and China have introduced a novel nanotechnology-based platform that can shorten the time required for COVID-19 diagnosis.
Choo’s team had developed a novel detection platform in which high-sensitivity SERS signals are produced by AuNPs uniformly arranged in the cavities of AuNDSs through a technique called DNA hybridization.
The current standard for COVID-19 diagnosis is reverse transcriptase-polymerase chain reaction (RT-PCR), a technique in which viral genes are detected after they undergo multiples cycles of amplification.